Sakshi Shekhar Mehta

6 Questions 12 Answers 0 Followers

Questions related from Sakshi Shekhar Mehta

Why do I see a Invalid NM tag error in the sorted bam files with bwa-mem2 command line?

I mapped my referencegenome using BWA-MEM2 and gave the output command for sorted bam files in the same command, after validating them with the GATK validatesamfile command which is as...

27 January 2025 3,287 1 View

Transformed (non recombinant) Vs Transformed (but recombinant) agrobacterium colonies?

Hi, it's been over a month that I am struggling with my clones. I got the desired clones for my study from some lab. They gave me the clones in the form of master plate. Now when I replated it and...

25 October 2024 6,693 6 View

Does anybody know if amino acids like GABA, BABA, proline, lysine, valine or any other non-aromatic amino acid are destroyed by UV light?

I am trying to eliminate infection from my plates and therefore, gave UV treatment after addition of amino acid (used for better growth). Can you please suggest if UV destroys amino acids or not,...

03 July 2024 3,132 1 View

How to get pure genomic DNA from CTAB method?

Hi, I have been trying to extract Plant genomic DNA via CTAB method and I am getting a 260/280 ratio ranging between 1.3 to 1.6. Any idea how to modify the protocol for fabaceae family plant...

04 June 2024 7,992 6 View

How to check a previously reported pair of primers?

Hi, I have few primers that are previously reported to amplify a conserved region. The ambiguity is that I want to be sure if they are correctly reported before ordering the same set of primers....

04 June 2024 4,213 3 View

Workshop or training on Bioinformatic analysis - Variant calling, RNA Seq analysis, Methylation patterns

Hi everyone, Its been over 3 months that I am trying to develop a script for variant calling and RNA seq analysis for my project. I have attended quite a few workshops but it feels like a scam....

01 January 1970 3,358 12 View