Saksham Gautam

2 Questions 2 Answers 0 Followers

Questions related from Saksham Gautam

Can I use CCCP instead of H₂O₂ as a positive control for ROS measurement through flow cytometry?

I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.

21 July 2024 6,354 0 View

What could be the probable reason for not getting any RNA product after IVT?

I am doing in-vitro transcription by T3 and T7 RNA polymerase to make a probe for ISH, but not getting any RNA product on 1.5% TBE gel. I am using a single digested pBluescript vector containing...

27 May 2022 2,174 3 View