Sakhila Ghimire

4 Questions 9 Answers 0 Followers

Questions related from Sakhila Ghimire

Why is my SPSS V25 acting crazy?

Hello everyone. I have been using SPSS for quite a time. I never had problems in previous times. But past couple of months, every time I fill information in my SPSS , some strange character...

09 December 2020 5,626 3 View

Does anyone know the neutrophil marker for qPCR in human gut (duodenum, ileum, colon) biopsies?

Hello everyone. I have collected intestinal biopsies from stem cell transplanted patients in RNAlater and wish to quantify neutrophils only via qPCR. As we know that the mucosa of gut is filled...

26 November 2020 2,875 3 View

Does anyone know how to stain Innate Lymphoid cells type 3 (absolutely ILC3s only) in human colon biopsy?

I am interested to find out the composition of ILCs 3 in colon of GvHD patients. Since I only have paraffin embeded tissues, all I can do is staining cells and taking to microscope. It would be...

11 March 2015 8,319 6 View

Can anyone help with a sybr green qPCR for hIL22?

I am trying to find out good standard for hIL22 using sybr green technique, but everything I tried gave bad slope, bad efficiency and multiple melting curve. Its hard to find good primers for...

13 May 2014 3,882 1 View