How to do JC-1 experiment for Mitochondrial membrane potentialMMP using isolated Mitochondria, Microplate Reader and fluorescence confocal microscopy?

I'm trying to find MMP of isolated chicken hepatocytes Mitochondria Infected with Salmonella Typhimurium using JC-1 dye. I want to ask, Do i have to use fresh isolated Mitochondria from Liver...

17 March 2021 3,768 4 View