Sajjad Ashraf

4 Questions 7 Answers 0 Followers

Questions related from Sajjad Ashraf

How to quantify total protein in the cell from western blot of Cytosolic and nuclear protein?

Hi, Anybody knows how can we quantify total amount of specific protein i.e X protein from western blot of cytosolic and nuclear fractions? Thanks in advance. Best.

08 September 2016 2,039 3 View

What is the composition of Chondrogenic medium?

Hello. I need to know the composition of chondrogenic medium. Can we use 10%FBS along with 1%ITS in chondrogenic medium? If possible can somebody give me reference article in which both of these...

12 December 2015 325 2 View

How can I interpret my consistent Luciferase results?

Hi, I have performed Luciferase assay more than 5 time separately. I transfected the 293T cells as well as HeLa cells with empty vector and vector harboring my gene of interest along with pGL3...

30 April 2015 7,311 3 View

Can we perform luciferase assay via overexpression of certain gene vector and also have gene sequence for GFP Protein?

Does somebody has experience of Luciferase assay?. Actually my gene of interest is present in a vector also harboring GFP sequence. So transfection of this vector also results in expression of GFP...

14 April 2015 6,152 5 View