Sajid Nisar

2 Questions 1 Answers 0 Followers

Questions related from Sajid Nisar

Why I am getting very bright primer dimers but faint colour PCR product?

Hi to all Hope you are doing well. I am amplifying Kan cassette in PkD4 plasmid with 40bp extensions in primers to knockout gene in MG1655 E.COLI by lambda red mutagenesis . the expected product...

22 January 2024 9,058 1 View

Why I got few very tiny colonies on negative control after tn5 transformation ?

Hi to all. Hope all of you are doing well. I am constructing Ez-tn5 (Kan2) transposon Library in E.coli to create mutants . I got ver very tiny colonies ( 15-20) on nagative plate also(water...

09 July 2022 4,002 0 View