Saira Ibrahim Khan

2 Questions 3 Answers 0 Followers

Questions related from Saira Ibrahim Khan

Can a power failure during the first spin of PBMC isolation ruin the buffy coat layer?

We have power trips going on quite frequently in our lab and to say the least this is a nightmare for my PBMC isolations. I was wondering if it would ruin the layer separation permanently or is...

08 February 2015 4,746 10 View

Can I refreeze Brefeldin A (bfa) once and use it in intracellular cytokine staining?

I'm aware it's not recommended to thaw and refreeze bfa but I prepared larger volumes of aliquots. I would really like to be economical and use it at least once after thawing, but I was hoping if...

29 December 2014 4,338 8 View