Saima Qayum

5 Questions 4 Answers 0 Followers

Questions related from Saima Qayum

What is the best way to do inter assay and intra assay validation of an assay?

I am doing realtime PCR for a known mutation.(a) Intra assay and (b)Inter assay validation are part of my experiments. According to my knowledge, Intra assay means running same sample mutliple...

10 October 2019 8,091 3 View

Doing NEXT GENERATION SEQUENCING, What is the minimum required reads of the targeted mutation in order to label a sample mutation positive?

I am doing targeted next generation sequencing targeting a number of somatic mutations. One of the mutations is a point mutation. Now in a sample if I have total 1000 reads, 10 reads are point...

10 October 2019 3,216 1 View

Does product size affect realtime PCR optimization?

I am optimizing a realtime PCR using probes with FAM and TAMRA. But I do not get any results. The product size is 77 basepairs. Is this small product aize the cause of failed optimization?what...

05 May 2019 8,638 4 View

Can i use Ambion nuclease free water Not DEPC treated for reconstitution of my primers and probes?

I intend to do a real time PCR using probes( FAM TAMRA). Can I use Ambion Nuclease free water Not DEPC treated to reconstitute my primers and probes?

02 February 2019 10,031 5 View

Can we make a stock of forward and reverse primers in a single tube?

I intend to do next generation sequencing for total six mutations in each sample. It involves 6 primer sets. In order to reduce the pipetting time can I make a stock of all primer sets in a single...

02 February 2019 9,471 4 View