Saieeda Fabia Ali

2 Questions 4 Answers 0 Followers

Questions related from Saieeda Fabia Ali

Does double orbital shaking in 96 plate reader effect eGFP fluorescence level? Why my control (without GFP) is showing same fluorescence as sample?

I am checking GFP fluorescence level in heme sensor. 200 ul of sample was loaded in black flat bottom 96 well plate in H1 Synergy machine. Temperature was 30 degree. I am using S. cerevisiae WT...

07 May 2024 5,717 3 View

Why my protein of interest is not inducing properly?

I am trying to express my protein of interest by IPTG induction. I can not see any difference between uninduced and induced sample in SDS-PAGE after induction for 4 hours in 28 degree. Final...

28 September 2022 4,789 6 View