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Questions related from Sai Supriya Avatapalli
The protein I am purifying needed an elution buffer with 2% Triton X-100. I formulated the elution buffer not keeping the CMC in mind. My goal is to make my protein sample triton free to check its...
01 January 2015 2,069 15 View
I recently came across this interesting software cellPACK by The Scripps Institute. Their website says, on Dec 3 2014, the software is made available to public. I couldn't really find a way to get...
12 December 2014 2,157 1 View
I've harvested IPTG induced E.coli BL21DE3 cells' suspension culture by spinning at 5000 rpm/15 min/4 degrees C. The pellet after spin looked pale pink colored. What could be the reasons?
11 November 2014 6,074 11 View
I had to digest my insert containing plasmid and another vector into which my insert has to be ligated, each with BamHI and NotI. Upon digestion, I ran the gel to check for results. The digestion...
07 July 2014 9,108 2 View
I wanted to amplify a 2kb gene with three domains, domain wise. The first domain was around 1 kb, the second was 400bp and the third had 300 bp. After PCR amplification, when I run the gel, I am...
07 July 2014 7,833 10 View
