Sai Cui

2 Questions 6 Answers 0 Followers

Questions related from Sai Cui

Is there any possibility for staining TLR4 only on cell membrane?

I need to do TLR4 staining on cell membrane. Every time I did it without any permeabilization, TLR4 in cytoplasm has also been stained. I have already tried PFA(4%) fixation with different...

07 July 2015 8,930 4 View

When I stain the membrane receptor of TLR4, why can I always see some signals from inside of the cells?

Hi, I am staining the TLR4 membrane receptor on ARPE-19 cells. I did not use any permeabilization, however, every time I can see some signals from inside of the cells. I think it is Golgi...

06 June 2015 8,848 7 View