What is the reason behind failure of Plasmid carrying gene optimization?

I have used primer for rmpA gene in hvK. pneumoniae. Primer sequence is rmpA F-P 5’-GGCCTAAAGCAGTTAACTGG-3’ and rmpA R-P 5’-CTAAATACTTGGCATGAGCCATC-3’. Recipe used are as follows Master mix 5...

12 December 2018 2,665 2 View