After cloning a single guide RNA in cas12, PCR product should be of 370bp but I am getting almost 570bp product. what would be the possible reason?

i have designed almost 5 single guide RNAs for CRISPR/cas12. After Gateway cloning, the possible PCR product should be almost 370bp and 4 single guide RNAs are giving the desired product. one...

14 April 2022 2,197 2 View