How one can decrease background noise in EMSA assay with SYBR Safe dye?

Hi all, I am performing an EMSA assay for RNA-Protein binding and I am using SYBR Safe dye. I have 0.75% agaros gel that contain 1X THE buffer and 100mM NaOAC. As you can see, I have lots of...

13 February 2024 4,418 4 View