Sahana Sheshachala

3 Questions 6 Answers 0 Followers

Questions related from Sahana Sheshachala

How to maximize the efficiency of SiO2-NH2 coupling with amine-DNA using gluteraldehyde as crosslinking agent?

I use the standard protocol of adding gluteraldehyde (50% in water, excess) to NH2-SiO2 in PBS 50 (7.4) for two hours and after purification adding NH2-DNA (excess) and overnight stirring. Later I...

16 September 2019 7,347 0 View

Am doing FITC coupling with APTES for FITC-APTES conjugate using ethanol as solvent. After 12h reaction, I got a precipitate, Is it normal?

I used FITC Isomer I and ethanol and APTES under argon. The precipitate is bright orange. I want to use this for synthesis of nanoparticles (dye conjugated) and the precipitate will not help in...

05 September 2019 3,303 2 View

How can I avoid the Cy5 dye degradation while synthesizing silica nanoparticles? Suggest some alternatives to Cy5 dye ?

I am using reverse micelle method to synthesize silica nanoparticles where in Cy5 -APTES (previously prepared) is covalently linked to the silica network to give fluorescent particles. I want to...

14 December 2018 2,921 6 View