2 Questions 4 Answers 0 Followers
Questions related from Sage Kim
Hello I am currently doing 96 well single colony selection using puromycin and DMEM with 10% FBS, 1% antibiotics. However, everytime i seed cells in 96 well plate and observe after 3 days, I see...
01 July 2025 5,978 4 View
I am transfecting RNAi and ASOs in PDACs mainly Panc-1, MiaPaCa-2 using lipofectamine 2000 according to the protocol. Upon western blot analysis, I couldnt observe any knockdown. I have tried...
22 January 2025 3,001 3 View