Sagar Sridhara

4 Questions 44 Answers 0 Followers

Questions related from Sagar Sridhara

Can we selectively promote protein crystallization against salt crystallization without a complete change of the crystallization condition?

Consider a crystallization condition that readily gives salt crystals on addition of protein. Is there a logical way to increase the probability of protein crystallization against the undesired...

04 April 2013 6,936 4 View

Can a protein crystallize after 2 months of its introduction to a particular well condition?

I was recently trying crystallization trials of my protein sample in a 96-well Corning plate. In one well of an experiment, some phase separations emerged after the 4th day of dispensing the...

01 January 2013 586 26 View

Can harvested bacterial cells (with recombinant protein inside) be flash-frozen using liquid nitrogen for long-term storage?

The E.coli cells with recombinant protein were harvested using the lysis buffer. I wanted to store the cells at -80 deg C for future lysis and protein purification. My main concern was that the...

12 December 2012 9,425 11 View

How long do you generally incubate the cell lysate to the chromatography resin to allow efficient protein binding?

With regard to heparin chromatography or IMAC, how long do you think is appropriate to allow maximum protein binding? I sometimes see quite an intense band of the desired protein in FT as well.

12 December 2012 4,663 14 View