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Questions related from Sagar Sridhara
Consider a crystallization condition that readily gives salt crystals on addition of protein. Is there a logical way to increase the probability of protein crystallization against the undesired...
04 April 2013 6,950 4 View
I was recently trying crystallization trials of my protein sample in a 96-well Corning plate. In one well of an experiment, some phase separations emerged after the 4th day of dispensing the...
01 January 2013 600 26 View
The E.coli cells with recombinant protein were harvested using the lysis buffer. I wanted to store the cells at -80 deg C for future lysis and protein purification. My main concern was that the...
12 December 2012 9,437 11 View
With regard to heparin chromatography or IMAC, how long do you think is appropriate to allow maximum protein binding? I sometimes see quite an intense band of the desired protein in FT as well.
12 December 2012 4,678 14 View