Sagar Gaikwad

5 Questions 42 Answers 0 Followers

Questions related from Sagar Gaikwad

Can anyone suggest the protocol for ex vivo stimulation of human whole blood?

Presently I am using the following steps: 1. I'm using freshly collected blood in EDTA- vacutainer tube. 2. Then, the blood was dilution at 1:3 ratio (blood: Media) in a cell culture plate...

09 September 2017 7,585 12 View

How is custom synthesis performed for small molecules?

I am planning to design and synthesis of novel small inhibitors/antagonist of TLRs. How should I begin? I have requested many private companies to provide samples but they could'nt provide as per...

03 March 2014 2,145 3 View

Can anyone recommend a protocol for Macrophage-T cell co culture experiment?

I wish to study T cell proliferation using Macrophage (adherent)- T cell (suspension) culture. After Ag treatment to Macrophage (24-48 hour), followed by fixing with 1% polyformaldehyde, I will...

12 December 2013 7,554 14 View

Can someone help me with a IL-1b ELISA problem in the detection or cells?

I am trying to assay the IL-1b from culture supernatant of mouse macrophage cell line but I'm not getting results. I am getting results with standard but not with samples, though I don't know...

05 May 2013 7,453 7 View

Can anyone recommend a protocol for semiquantitative RT-PCR?

Presently I am using the following steps: 1. Total cellular RNA extraction using the Trizol method. 2. Estimation of RNA using Nanospect. 3. One microgram of RNA used for reverse transcription...

03 March 2013 1,980 53 View