Sagar Gaikwad

2 Questions 29 Answers 0 Followers

Questions related from Sagar Gaikwad

Can anyone recommend a protocol for semiquantitative RT-PCR?

Presently I am using the following steps: 1. Total cellular RNA extraction using the Trizol method. 2. Estimation of RNA using Nanospect. 3. One microgram of RNA used for reverse transcription...

25 March 2013 2,847 51 View

Can any one suggest the exact protocol for NO assay using Griess reagent? Do I have to use colorless media for plating the cells?

At what condition do I have to treat cells with LPS? In serum free media? For how many hours?

05 February 2013 669 16 View