Safiulla Basha Syed

3 Questions 6 Answers 0 Followers

Questions related from Safiulla Basha Syed

Is a non-specific protein getting pulldown in my Co-IP experiment? If it is then how do I avoid this protein?

I am trying to pulldown a protein of 300 kDa using protein G agarose beads. I used two negative controls, one pulldown with just beads (without primary antibody) and another pulldown with another...

14 July 2019 3,374 8 View

Can anyone provide information on how to maintain my animal cell culture?

We have been handling animal cell cultures for a long time. Recently, we are facing a problem which we never faced earlier. The problem is that the cell morphology looks pretty good, media is also...

04 September 2014 1,844 11 View

How to model a template protein that has two chains into a single chain target?

I have a protein which has two chains each contains 422 residues, both chains are identical. I want to model this protein but the target sequence which I have contains more than 850 residues. When...

04 August 2013 4,187 3 View