Safeena Majeed

2 Questions 5 Answers 0 Followers

Questions related from Safeena Majeed

What is the optimum score for self-complementarity and self 3' complementarity for designing primers?

I have designed primers in NCBI primer BLAST. What would be the lowest score for self complementarity of the primers since I am getting 4 and 6 for forward and reverse respectively?

20 July 2021 3,895 5 View

How to obtain better amplicons in the reamplification of the PCR product?

Does the reamplification of the PCR product need any alterations in the original PCR components and procedure to get better and reliable amplicons? Also how best the issue of insufficient quantity...

03 July 2021 7,887 10 View