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Hi! I am trying to find out the complete genetic sequences of Odorant Binding Protein and Heat Shock Protein for an insect on NCBI nucleotide database, but I always end up with their partial mRNA...
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For the amplification of my target gene, I ran a Gradiant PCR by keeping all other components of the PCR mixture constant, and only altering the Tm. I set the Tm values at 52°, 53°, 54° and 55°....
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