Can anybody help me in ZO-1 staining in retina flatmount?

I tried to do ZO-1 (1:50) staining in C57bl/6 retina flatmount. and as secondry antibody I used goat anti rabbit IgG conjugated alexa fleur 488 (1:500) also for blocking I used 0.1% triton X-100,...

22 March 2018 2,575 5 View