Why KLH aggregates after adding with MBS in the conjugation?

I'm trying to make a conjugation carrier with KLH-MBS. My protocol is as: 1. 5mg of KLH+ 500ul of 10mM phosphate buffer (pH=7.2) 2. 1.5mg of MBS+ 100ul DMF (>99% purity) 3. 70ul of MBS from "2" is...

22 March 2018 165 3 View