Sachin Teotia

12 Questions 35 Answers 0 Followers

Questions related from Sachin Teotia

Is anyone interested to submit a book chapter in Springer Nature book on "“RNA-based technologies for functional genomics in plants”?

We are editing a book on “RNA-based technologies for functional genomics in plants” (Eds: Guiliang Tang, Sachin Teotia) for the Book series "Concepts & Strategies in Plant Sciences" with Springer...

03 March 2019 2,841 1 View

Why the cDNA not coming good from a good RNA?

I have isolated plant RNA from Qiagen kit. The 260/280 and 260/230 ratio are all above 2, the RNA looks good on the gel, then when I make cDNA and do real-time, 2 out of 6 samples show nothing on...

04 April 2016 9,514 13 View

Which is the best freely available software for analyzing Real time PCR data by ∆∆Ct method?

I have done qPCR on several genes along with the reference gene for mutant and wild type samples. I have got the Ct values. Which is the best free software where I can feed the Ct values and get...

03 March 2016 8,014 10 View

How can I increase the yield of longer capped transcripts for in vitro transcription?

I am setting up in vitro reaction by T7 polymerase on linearized plasmids purified by Promega column after digestion. Some people recommend using phenol;chloroform to clean DNA before...

10 October 2015 2,672 6 View

Is it possible to silence a transgene (driven by a strong promoter) by an artificial miRNA driven by an inducible promoter in Arabidopsis?

While in literature there is no report, to my knowledge, to silence one transgene in Arabidopsis (under 35S promoter) using another transgene (artificial microRNA, under inducible system). I think...

06 June 2015 2,521 1 View

Is the trizol method to isolate RNA from plant tissues good for RNA-seq experiments?

We normally isolate RNA from rice and Arabidopsis by using trizol. We would like to do RNA-seq and small-RNA seq. There are commercial kits like miRVana which allow to isolate total RNA including...

03 March 2015 745 5 View

Through VIGS based system, can any big protein spread from the leaves to the germline tissues?

If we introduce any big protein (for example about 1300 amino acids) infiltrated through VIGS system, can it spread from the leaves and reach the germline cells? Is there any report of it? We have...

03 March 2015 5,690 1 View

While doing VIGS, can the infiltrated virus spread from the leaves to the germline tissues and knockdown the germline gene?

We have seen VIGS induced silencing of PDS, GFP and many other genes in many species. Is there any report or is it possible that we can knockdown a gene expressed in germline tissues using VIGS?...

01 January 2015 7,688 3 View

Which is the best kit to isolate plant total RNA (inclucing miRNA)?

I want to isolate total RNA (including siRNA and miRNA) from Arabidopsis seedlings. We want to do RNA-seq and small RNA-seq with that RNA. There are few kits available for this purpose to isolate...

09 September 2014 1,553 14 View

Is pTF101.1 binary vector good to transform Arabidopsis?

I want to transform Arabidopsis with pTF101.1 binary vector which has a bar resistance gene. Normally people have used this vector for crops such as soybean and maize, not frequently been used for...

09 September 2014 4,354 4 View

What is the right hygromycin concentration to select rice (Nipponbare) calli?

Hi, I am selecting rice calli of Nipponbare on hygromycin (20mg/L) on selection as well as regeneration media. Some protocols suggest more than 30mg/L and even 50. What is the optimal...

09 September 2014 8,403 15 View

Does anyone have experience deciding parameters for RNA-seq on Arabidopsis?

I have a question for doing RNA-seq on Arabidopsis samples comparing differential gene expression between wild type and miRNA mutant lines. Just one mutant will be compared.How many replicates are...

08 August 2014 536 2 View