Sachin Surwase

4 Questions 8 Answers 0 Followers

Questions related from Sachin Surwase

How to perform B16F10-OVA stable cell line selection with the help of 418 (Geneticin) ?

I am growing a Melanoma cell line expressing Ovalbumin (OVA) protein. I am looking for maintaining the stable expression of the protein by cell line and the researcher use G418 with a variable...

16 March 2021 2,127 2 View

How to concentrate the plasmid DNA ?

I am having 40 ng/ul of plasmid DNA and would like to concentrate it to get at least 10 to 20 times concentrated solution 400 ng-800 ng/ ul. Centrifuge by AMICON ? Freeze dry? Precipitation...

11 March 2021 6,361 5 View

Is it possible to produce mucus secreting stable cell line HT29-MTX from HT29 colon carcinoma in a lab?

Hello, I am looking for a mucus-secreting cell line called HT29-MTX (E12 cell line). Our local cell bank can provide the HT29 cell line but not the HT-29-MTX cell line. Can anyone help with...

06 March 2021 2,317 0 View

How we can avoid caco-2 cell aggregation during cell count and seeding into well ?

Growing caco-2 cells, but can not get cell count due to aggregation of cells (bunch of 10-20 cells). Already tried pipetting and vortex but still there are cell agrregate making it difficult to...

20 March 2019 6,099 3 View