Sachin Aryal

3 Questions 6 Answers 0 Followers

Questions related from Sachin Aryal

Is it a problem with the gel run?

I want to check the mTORC activity and for that I am using rat kidney. I used cell lytic buffer from Sigma Aldrich to extract the proteins from rat kidney (50mg sample homogenized in 200ul cell...

28 February 2024 9,166 3 View

Any particular way of stripping and reprobing antibodies in the same target?

I am working with histone sub-unit 3 lysine 9 position (H3K9) and looking for particular modifications that are happening there (beta-hydroxybutyrylation and butyrylation). I used H3K9BHB as a...

04 January 2023 6,885 3 View

I am not able to get good bands for the rat brain samples. It looks leaky and inconsistent as seen in the picture below. Can anyone help?

I followed the exact protocol for kidney, liver samples and it worked. But it is not working for brain samples. I extracted the histone proteins using pre-lysis, lysis, and balanced buffer from...

18 October 2022 8,917 9 View