Sabawoon Nisar

3 Questions 1 Answers 0 Followers

Questions related from Sabawoon Nisar

Title: Understanding T/S Calculation in Cawthon’s MMqPCR Method for Telomere Length Measurement?

Hello everyone, I am new to qPCR-based methods and trying to understand how the monochrome multiplex quantitative PCR (MMqPCR) method, developed by Cawthon (2009), calculates the T/S ratio for...

11 March 2025 2,630 1 View

Issue with PCR Cycles Not Initiating Using Cawthon's Monochrome Multiplex PCR Method on Bio-Rad CFX96. Any advice?

I’ve been trying to calculate relative telomere length using Cawthon’s monochrome multiplex PCR method on a Bio-Rad CFX96™ Real-Time PCR Detection System. However, when I attempt to run the PCR,...

04 February 2025 615 1 View

Urgent: How long can I keep cells in an eppendorf tube (for DNA extraction) while i do cell counting?

I have to extract DNA from 1.5 ml of fresh cancer cells (fibroblasts cultured in MEM), but before that i need to count the cells. I need to do both on the same day. My question is how long can i...

28 September 2024 7,071 2 View