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Questions related from Sabahat Fatima
I have done RT-PCR using input RNA concentration of 400ng. Then I use 2uL of cDNA in 20uL reaction volume. I obtained Ct in range of 22-23. But now I am getting late Ct for a different sample with...
07 November 2024 3,435 1 View
I am getting a very late Ct value for U6. The RFU are also low. I am using stem loop primers for cDNA synthesis (specific to U6), Forward primer and Universal Reverse Primer. I am also attaching...
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