Saaraj Gupta

7 Questions 3 Answers 0 Followers

Questions related from Saaraj Gupta

Ni-NTA protein purification - how to obtain higher yield concentration?

Hello, I am trying to purify a 6X-His tagged protein using Ni-NTA chromatography. However, I obtain very less yield & concentration; and considerable protein in load flow through. Any...

17 June 2024 5,722 5 View

Has anyone tried dissolving PVA (poly vinyl alcohol) in water?

I am trying to dissolve PVA in water and even after boiling it does not dissolve. Is there any specific method to dissolve it in water? I am trying to make a 10% PVA solution in water.

15 September 2023 3,085 0 View

Has anyone used 'Kamoer' peristaltic pumps in lab setting?

Hello, for liquid transfer/ filtration - has anyone used peristaltic pumps of 'Kamoer' make? I am looking for some user reviews before I buy it.

31 May 2023 1,390 0 View

How to generate polyclonal antibodies in animal against human IgM?

Hello all, I wish to understand the procedure for generation of polyclonal antibodies against human IgM. How to purify human IgM from serum? How to prepare crude IgM preparation for animal...

11 July 2022 9,156 1 View

Selectively visualize his-tag fusion proteins

I need to know if there's a way I can specifically quantify (visually) his-tag fusion protein. I have checked for commercial his-tag stain reagents, but I'm looking for state-of-art method(s)....

20 May 2013 7,768 3 View

Can someone help me with DNA polymerase activity analysis?

I've expressed Taq polymerase in E.coli. I have found a few reports for its purification also using sephadex columns. Next, I need to check its efficacy preferably by methods other than PCR. Can...

17 May 2013 2,786 2 View

How to separate/purify B lymphocytes from blood/spleen?

I am looking for cheaper and still efficient state of art methods. I have checked for FACS and a few commercial kits but they are out of my budget.

04 March 2013 4,448 7 View