How to transfect hippocampal neurons at DIV2 without the cells clumping together or fasciculating?

I am working with primary hippocampal cells from E18 rats. The problem I am facing is with the transfection of these neurons in a 12-well plate with Lipofectamine 2000. When I transfect at DIV 2...

30 April 2024 8,004 0 View