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In my research half-life of peptide is 10h in E.coli despite it`s optimum expression is 7h after induce, how does its expression fulfill?
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Peptides are about 6.8, 3, 2.9 and 2.7 KDa and I use Tris-Tricine SDS-PAGE (16.5% and 18%) but gel resolution is not clear. so I can′t see 5 and 3.4 KDa bands of marker. could anyone help me with...
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