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I transfected px458 in Expi293F HEK cells with lipofectamine and then sorted single cell by FACS into 96 well plates. The medium is Expression medium without antibiotics. The cells are cultured in...
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I know that efficiency of transfection for primary B cells is very low with Lipofectamine. But I did not try that for EBV immortalized B cells. Do you have any experience with transfection of EBV...
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