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Does anyone know why TFA (ion pairing agent in HPLC) is better for TiO2 and Phosphopeptides? I am doing SILAC experiment on a kinase module of mediator, and I would like to get some inputs...
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I am working on Phosphoproteomics experiment by using SILAC (on S. cerevisiae). I have got back my MS data and was suggested to use Proteome Discoverer 2.2 to analyze the data. Since this is my...
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