Ryan Skiba

2 Questions 1 Answers 0 Followers

Questions related from Ryan Skiba

How can I purify filamentous fungi from leaf tissue?

I want to perform PacBio RNA-seq of a filamentous fungal isolate during multiple infection time-points in barley leaf. I was told that PacBio RNA-seq would not be viable due to the low...

13 June 2024 3,914 1 View

Is there an upper limit on the size of a genomic fragment that can be eliminated using a split-marker PCR approach?

I am trying to knock out a gene involved in virulence from a genomic region of roughly 150kb. So far I have been using CRISPR to target individual candidate genes, but I have recently exhausted my...

14 June 2021 4,639 1 View