Ryan Rahim

2 Questions 7 Answers 0 Followers

Questions related from Ryan Rahim

How do you plate an MTT Assay when your IC50 is between 25 and 12.5 micromolar?

I am doing an MTT Assay of a new drug that I am experimenting with on MDAMB-231 Cells; however, I cannot figure out a dilution factor or an experimental setup on the MTT assay to see the IC50...

17 February 2022 2,191 5 View

Is there a way to better culture the MCF12/10A cells?

I am having trouble with the MCF10A cells that I am working on. Some are sticking to the bottom of the plates but they are not growing and there is always debris in the plate after 3-4 times of...

03 November 2021 1,427 6 View