Ryan S Pawell

2 Questions 5 Answers 0 Followers

Questions related from Ryan S Pawell

How do you detect cytosolic delivery of a plasmid?

We are working on an intracellular delivery technology and have verified cytosolic delivery of a membrane impermeable dye and dextrans, but are having trouble with GFP plasmids and need to check...

11 April 2016 8,477 1 View

Can DAPI, Calcein AM and Propidium Iodide be used to (1) locate and (2) measure viable or (3) viable and permeabilised (transfected) cells?

We are developing an assay to measure an analogue for cell transfection. In theory, DAPI (blue) allows us to locate all cells, Calcein AM (green) indicates viability and Propidium Iodide (red)...

17 December 2015 5,637 4 View