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I am measuring the binding of thrombin binding aptamer (TBA) to thrombin using fluorescence anisotropy. TBA is labelled with a fluorophore (5'-FAM) and was initially kept at a constant...
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A protocol I have been sent for an ELISA-type experiment uses 0.05% (v/v) Tween-20 in PBS as a detergent to remove weakly bound proteins from a microtiter plate. Since we do not have Tween-20, can...
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