What is the best approach for freezing and thawing HUVECs?

I froze some HUVECs in 10% DMSO and 90% FBS over a year ago. When defrosting I placed in a water bath until mostly thawed, then added warmed EGM-2 to cryotube before transferring to a 15 mL Falcon...

28 April 2015 7,226 6 View