Ruslan Kramor

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Questions related from Ruslan Kramor

What concentration of Zeocin or its analog CL990-cin do you use for Pichia pastoris transformants selection?

At first I used for my transformations by electroporation CL990-cin in concentration 100 mkg/ml. But single colonies of not-transformed strain grow on a plate. I increased concentration of...

05 November 2020 4,870 2 View

Do I need to do codon optimization for heterologous protein in Pichia pastoris if i don't know hosts codon usage?

Hello! I need any explanation. I have to express plant protein in Pichia pastoris, but the codon usage of hosts plant is unknown yet ( i searched for it on kazusa). I found several articles on...

16 October 2020 3,853 5 View