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I expressed the recombinant protein and purified with Ni-NTA in pure form (Single band). I set up the dialysis to remove urea and imidazole from the purified protein sample (10ml). After dialysis,...
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The protein size is 14kDa. I am purifying my protein by gel elution after excising the appropriate band. Previously, I have seen the single band at 14kDa position but after keeping it in 4 degree...
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