Rupendra Shrestha

2 Questions 5 Answers 0 Followers

Questions related from Rupendra Shrestha

How to differentiate iPSC morphology whether it is full or partial reprogrammed?

I have generated the iPSC using SeV [imaged attached]. The iPSC is positive for Tra-1-81. I picked the cells manually for further expansion but my cells neither attached nor expand. I have gone...

28 February 2017 3,009 7 View

How can a 14 kDa protein be missing during dialysis?

I expressed the recombinant protein and purified with Ni-NTA in pure form (Single band). I set up the dialysis to remove urea and imidazole from the purified protein sample (10ml). After dialysis,...

24 December 2015 4,644 8 View