Rupendra Shrestha

3 Questions 6 Answers 0 Followers

Questions related from Rupendra Shrestha

How to differentiate iPSC morphology whether it is full or partial reprogrammed?

I have generated the iPSC using SeV [imaged attached]. The iPSC is positive for Tra-1-81. I picked the cells manually for further expansion but my cells neither attached nor expand. I have gone...

28 February 2017 3,036 7 View

How can a 14 kDa protein be missing during dialysis?

I expressed the recombinant protein and purified with Ni-NTA in pure form (Single band). I set up the dialysis to remove urea and imidazole from the purified protein sample (10ml). After dialysis,...

24 December 2015 4,665 8 View

Have you noticed increased spam activity on ResearchGate, such as irrelevant comments asking for email on your papers?

Alert for Researchers Dear researchers, be cautious of spam on ResearchGate, including irrelevant comments, requests for your email or time, self-promotion, or harmful links. Such activity can...

01 January 1970 9,444 4 View