Ruonan Gao

3 Questions 5 Answers 0 Followers

Questions related from Ruonan Gao

Why no IL18 production in BV2 after LPS treatment?

I'm trying to induce inflammation in BV2 cell line. I seed 4*10^5/well cell in 6-well plate, add 1ug/ml LPS for 4 or 23.5 hours, then add 10uM nigericin for 30 minutes. I can detect NLRP3...

08 June 2023 7,136 4 View

Can LPS induce inflammation in HEK293 cells?

I'm trying to use LPS to induce inflammation in HEK293 cells.I've tried different LPS concentration and incubation time, but qPCR result showed no difference in cytokine expression. Does anyone...

01 July 2022 6,849 2 View

NLRP3 expression not change in THP1 after LPS stimulation

I'm trying to induce inflammasome activation in THP1. I added 100ng/ml PMA to induce differentiation of THP1 for 24 hours, then change medium without PMA and rest for 24 hours. I added 1ug/ml LPS...

01 January 1970 4,880 3 View