Ruojin Tian

2 Questions 12 Answers 0 Followers

Questions related from Ruojin Tian

To clone the toxic gene: Colony PCR shows good bands, but after I extract the DNA I cannot see the bands anymore?

Hi everyone, I have a very troublesome time when I trying to build the construct of my gene. Since the gene sequence of my target insert is toxic due to the leaky expression, I used the competent...

14 July 2021 7,499 15 View

Why there is a 11 kDa band showing in SDS-PAGE of my IgG1 digestion with Papain?

Hi guys, I am using the papain digestion system to abtain a Fab. Here is my recent result, I got thin bands around 55, 45; thick bands around 30, 25, which may indicate incomplete digestion, but...

28 June 2020 4,004 9 View