RunQing Yang

4 Questions 10 Answers 0 Followers

Questions related from RunQing Yang

Did anyone have used western to detect CD3ζ chain phosphorylation?

I try very hard to detect CD3ζ chain phosphorylation, but until now, I still can‘t get anything. Here is my TCR stimulation protocol: 1. I resuspend the thymocytes at 1x 107 cells/mL in...

12 September 2016 2,162 3 View

Does anyone know how to induce T cell anergy in vivo?

I give high does(500ug, i.v.) ova peptide to induce OT II mice in vivo, but this method doesn't work. And I still don't know why. I would be very grateful it anyone could tell me how to do this...

22 January 2015 8,358 5 View

Why doesn't the immune system develop CD8+ Treg? Why Treg is always CD4+?

As we all know, nTreg develops in thymus, but considering that CD4, CD8 all develop from thymus, it's so strange that CD8+ T cells don't develop Treg.

05 September 2014 4,839 4 View

Does anyone have any experience in inducing Treg in vivo? What factors should be taken into consideration?

I isolate naive OTII CD4+ T cells(CD45.2) and transfer these cells into B6 mice (CD45.1), The next day, I give 10 ug OVA peptide(control group gives PBS) by i.v. injection. 5 days after antigen...

01 September 2014 9,539 6 View