Rumela Bose

2 Questions 4 Answers 0 Followers

Questions related from Rumela Bose

Why my HSCs are dying after sorting, even after sorting at 1-5 flow rate and collecting them in suitable media?

I am isolating HSC (hematopoietic stem cells) from mice and sorting them with standard antibodies in a BDS8 sorter. I also use a viability dye to gate for live cells. Typically we get 5000-6000...

30 June 2025 599 5 View

Why is the reading for renilla luciferase (endogenous control) lower in miRNA over-expressing cells compared to the scramble cells?

I have cloned the 3'UTR of my gene in the pmirGLO vector from Promega and using Neuro2A cells for co-transfecting the GLO-3'UTR plasmid (100ng) and miRNA mimics and inhibitors (50nM each)....

17 March 2025 8,568 0 View