Rukhsana Kausar

2 Questions 1 Answers 0 Followers

Questions related from Rukhsana Kausar

Can anyone help with a Luciferase assay?

Hi, I am doing luciferase assay. I am using HCT116 and transfected by using Lipofectamine. I checked luciferase assay with only pGL3 Basic (2ug) and then performed luciferase assay according to...

25 August 2014 1,040 5 View

What is the problem when I try to amplify 1.1kb promoter region from HEK293 genomic DNA.

Previously I successfully amplified 1.7kb and 1kb using same method. My Primers are 30nt in length and Tm=79 and 80. I used 200ng of gDNA. My PCR condition is 94 for 5m, 94 for 30sec. Annealing...

04 June 2014 2,015 4 View