How to pick gRNAs and design donor DNA for fluorescent tag CRISPR KI (Knock-In) in U2OS?

I have never done any CRISPR experiment before and my tutor asked me to use CRISPR KI to induce a mCherry tag for a gene in U2OS, I decided to follow the protocol described in Generation and...

19 September 2024 7,743 0 View