Ruiqi Li

4 Questions 2 Answers 0 Followers

Questions related from Ruiqi Li

Suggestions for high molecular weight protein western blotting?

Hi I am trying to detecting ZO-1 (~210 kDa) in colon tissue. I used 1 hr transferring to the blot and I am pretty sure I wash the membrane very well after secondary antibody and HRP. But I still...

02 June 2017 4,906 3 View

Protein degradation in colon tissue stored in -80 C?

Hi Guys, I am working on western blotting of tight junction proteins in mice colon tissue. Currently, I suspect my problem is low protein content in the prepared lysate. I am just wondering...

15 April 2017 366 1 View

BCA over estimate protein content in tissue prepared with RIPA buffer?

Hi, I use BCA determine protein content in mice colon tissue sample prepared using RIPA buffer supplemented with protease and PMSF. I suspect that the BCA highly over estimated the protein...

15 April 2017 6,070 3 View

How to prepare fecal sample for measuring short chain fatty acids, is it necessary to derivatization?

I am trying to determine the short chain fatty acids in fecal sample from mice using GC. I read some papers, some of them just homogenize the fecal and filter, some of the papers do a...

09 June 2016 931 3 View