Rui-Gang Xu

3 Questions 7 Answers 0 Followers

Questions related from Rui-Gang Xu

What can I do if no Tm shift was observed in thermo shift assay when adding ATP analogues to ATPase protein?

Hi, I am doing thermo shift assay for one protein (It has an ATPase domain, thermostable, soluble) with 5mM to 15mM different ATP analogues(including ATP, ADP, ATP-gamma-S, etc) to see if I could...

01 January 2015 4,034 7 View

When my protein is frozen by liquid nitrogen I lose diffraction in crystallization, why?

I am working on a thermostable protein (host living temperature at 60). After the purification, I set up some trays using fresh purified protein and got crystals diffract to about 3.5A....

10 October 2014 2,866 6 View

How can I purify protein from lysate containing 1M arginine-hydrogen chloride?

My protein is almost insoluble without high concentration of arginine-hydrogen chloride adding to the cell lysate in the expression test. It cannot bind to the Nickel affinity column with high...

07 July 2014 10,021 6 View